Composite

Part:BBa_K4607013:Design

Designed by: Axel Rojero   Group: iGEM23_Tec-Chihuahua   (2023-09-09)


Expression cassette for PCNP-CecA-LysSS protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 833
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
    Illegal SpeI site found at 814
    Illegal PstI site found at 505
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 833
    Illegal SpeI site found at 127
    Illegal SpeI site found at 814
    Illegal PstI site found at 505
    Illegal NotI site found at 975
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 833
    Illegal BglII site found at 30
    Illegal BamHI site found at 827
    Illegal XhoI site found at 984
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 833
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
    Illegal SpeI site found at 814
    Illegal PstI site found at 505
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 833
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
    Illegal SpeI site found at 814
    Illegal PstI site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for expression in Escherichia coli.


Source

This part is composed of the following biobricks:BBa_K4607008, BBa_K4607009, BBa_K4607010

References